The chromatograms come off the machine with all bases in upper case. This opens the file in Chromas (see below under installation notes if some other program opens it instead of Chromas). MEGA also has an alignment editor, but I’ve not really used it very much.ĭouble click on the chromatogram file (usually has the extension ab1). BioEdit can also edit chromatograms, but I find Chromas to be nicer. If I loose my sequence alignment, at least all my chromatograms with the correct edits are still there to rebuild it from. Guide to editing sequences with Chromas and BioEdit Guide to editing sequences with Chromas and BioEditīy Peter Unmack Editing chromatograms with ChromasĬhromas has the advantage the you can save all of your chromatograms which can subsequently be used in any other programs unlike Sequencher which saves everything in a project file which cannot be opened by anything else.
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